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Molecular cloning steps in order include:

A、extractionofvectorDNA,ligationoftargetDNAtovectorDNA,restrictionoftargetDNA;
B、restrictionoftargetDNA,ligationofcut(thatis,restricted)targetDNAtovectorDNAwhichhasalreadybeencut,transformationofhostcells;
C、cuttingtargetDNA,cuttingvectorDNA,amplifyingtargetDNA;
D、amplifyingtargetDNA,ligatingtargetDNAtocutvectorDNA,homologousrecombinationoftargetandvectorDNA

发布时间：2026-01-14 05:05:43

推荐参考答案 ( 由专技宝官方老师解答 )

答案：restriction of target DNA, ligation of cut (that is, restricted) target DNA to vector DNA which has already been cut, transformation of host cells

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